Tilapia Lake Virus (TiLV) from Vietnam is genetically distantly related to TiLV strains from other countries
28/03/23 04:30PM
Tran, Triet Hanh; Vy Thuy Hoang Nguyen; Hieu Chi Nguyen Bui; Yen Binh Thi Tran; Huong Thanh Thi Tran; et al. Journal of Fish Diseases; Oxford Vol. 45, Iss. 9, (Sep 2022): 1389-1401. DOI:10.1111/jfd.13669
Abstract:
Tilapia Lake Virus (TiLV) is reported as a threat to
tilapia aquaculture in 16 countries on four continents with outbreaks causing
up to 90% mortality. This research is one of the first studies on TiLVs from Viet Nam. We propagated successfully
one TiLV isolate HB196‐VN‐2020 from a diseased tilapia sample using an E‐11
cell line and evaluated its virulence in two different weights of red hybrid
tilapia and three serial 10‐fold diluted viral titers. Smaller fish (4.5 ± 1.98 g)
were proved to be more susceptible to TiLV infection at the viral titre of
9.1 × 105 TCID50 fish−1 than larger fish (20.8 ± 7.5 g) with
the mortalities of 92.5% and 12.5%, respectively. Reassortant detection
analysis revealed seven potential reassortment events among 23 TiLV genomes,
indicating the mixed infection of multiple TiLV isolates at the farms and the
fish movement among different regions. Seven maximum likelihood phylogenetic
trees based on the individual segments or the concatenated coding regions of
some segments showed the genetically distant relationship of the Southern
Vietnamese isolate RIA2‐VN‐2019 with the 21 reference isolates, and suggest the
different origins of two Vietnamese TiLV isolates (RIA2‐VN‐2019 and
HB196‐VN‐2020). However, additional sequences from various sampling locations
and times are required to better understand the impacts of genetic diversity
and reassortments on the evolution, migration and natural selection of TiLVs in Viet Nam and other countries.
Fulltext: https://doi.org/10.1111/jfd.13669
(Source: https://www.proquest.com/scholarly-journals/tilapia-lake-virus-tilv-vietnam-is-genetically/docview/2700262222/se-2?accountid=28030)
