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Abstract
Fishery by-products (FBPs) have been increasingly
investigated for the extraction and production of a vast array of active
molecules. The aim of this study was to produce phenazine compounds from FBPs
via microbial fermentation and assess their novel antinematode effect. Among
various FBPs, squid pen powder (SPP) was discovered as the most suitable
substrate for phenazine production by Pseudomonas aeruginosa TUN03
fermentation. Various small-scale experiments conducted in flasks for phenazine
production indicated that the most suitable was the newly designed liquid
medium which included 1% SPP, 0.05% MgSO4, and 0.1% Ca3(PO4)2 (initial pH 7).
Phenazines were further studied for scale-up bioproduction in a 14 L bioreactor
system resulting in a high yield (22.73 µg/mL) in a much shorter cultivation
time (12 h). In the fermented culture broth, hemi-pyocyanin (HPC) was detected
as a major phenazine compound with an area percentage of 11.28% in the crude
sample. In the bioactivity tests, crude phenazines and HPC demonstrate novel
potential nematicidal activity against black pepper nematodes, inhibiting both
juveniles (J2) nematodes and egg hatching. The results of this work suggest a
novel use of SPP for cost-effective bioproduction of HPC, a novel potential
nematodes inhibitor. Moreover, the combination of MgSO4 and Ca3(PO4)2 was also
found to be a novel salt composition that significantly enhanced phenazine
yield by P. aeruginosa fermentation in this work.
Fulltext: https://doi.org/10.3390/fishes7030113
(Source: https://www.proquest.com/scholarly-journals/utilization-fishery-processing-product-squid-pens/docview/2679715985/se-2?accountid=28030)