Abstract: The ecological response of nematode communities to dam construction has limited attention. In this study, the response of intertidal nematode communities in the Ba Lai River (Mekong Delta, Vietnam) to the construction of an irrigation dam was investigated. Nematode communities and environmental parameters were investigated during the rainy season of 2015. The obtained results showed that the Ba Lai dam had caused negative impacts on the local environment by disrupting longitudinal connectivity, the accumulation of nutrients (total organic carbon, total nitrogen, total phosphorus), and heavy metals (copper, iron, arsenic, lead) in the upstream and the lateral sides of the dam, consequently leading to changes in the nematode communities. The response of nematode communities to the dam's presence was clear based on their abundance, diversity, dominant genera, and community composition. Furthermore, changes in the abundance and diversity of nematodes in the Ba Lai River appeared to be controlled primarily by acidity (pH), total suspended solids (TSS), iron (Fe), clay, and salinity most responsible for changes to nematode communities. Because the nematode communities are well adapted to the physicochemical disturbances caused by dam construction, they are a potential tool for ecological monitoring and understanding the influence of dams on aquatic ecosystems.