Abstract: The lowlands of Southeast Asia are rich in biodiversity and many are being drastically altered through rapid urbanization. There are few remaining pristine ecosystems to support amphibian species that depend on these lowlands, and human-dominated landscapes contain an increasing proportion of potential habitats. We studied amphibian communities and associated habitat characteristics of water bodies in three land-use types (urban, suburban, and rice paddy) in each of three large metropolitan areas (Hanoi, Da Nang, and Ho Chi Minh City). Each of the 12 amphibian species documented was found in all three land-use types. Although all species were present in urban habitats, their occurrence, alpha diversity, and abundance were lower there compared with suburban areas or rice paddies. Individual species showed differential responses to habitat characteristics, and species richness was positively associated with presence of aquatic vegetation, presence of shallow areas, amount of surrounding vegetated uplands, and banks constructed of soil rather than concrete or rock. Urbanization tended to decrease all of these associated features. During the 10 months of this study, at least 14% of the surveyed urban and suburban water bodies were altered in ways that greatly or entirely reduced amphibian breeding. However, simple habitat enhancements could be beneficial; half of the species we found bred in concrete fountains that included potted ornamental plants and that were located in manicured parks in the center of Ho Chi Minh City. Although continued urbanization threatens native amphibians, cities are able to support many species where key habitat features are present.